Alveolar Macrophage were collected at the time point of 1h, 6h and 12h by the means of bronchoalveolar lavage ( BAL), and its TLR2/ 4 mRNA and protein were detected with Flow Cytometry and Real-time PCR. 于再灌注1h,6h,12h后通过支气管肺泡灌洗获取肺泡巨噬细胞,采用荧光定量PCR及流式细胞学检测其TLR2/4mRNA和蛋白的表达。
Cover the alveolar of A and B group with titanium membranes, fix four point with small titanium screw and suture contralateral gums tightly. 在A、B两组牙槽嵴顶覆盖钛膜,四角用小钛钉固定,严密缝合牙龈。